Modeling domain formation of MARCKS and protein kinase C at cellular membranes

EPJ Nonlinear Biomedical Physics

Table 1 Parameter values of the model

Molecule	Parameter	Value	Meaning	Ref.
MARCKS
	[M R K]	7.5 μ M	Concentration	[4]
	[M R K]_o	15 μ M	Coverage	[29, 30]
	k b mrk	4 ×10³ s ^-1	Binding	[23]
	k u mrk	0.1 s ^-1	Unbinding	[23]
	k pkc mrk	0.25 μ M ^-1 s ^-1	Phosphorylation	[23]
	k _mrk	0.15 μ M	Michaelis-Menten binding	[15]
	k ppa mrk	0.03 s ^-1	Dephosphorylation	[9]
	D _c	5 μ m ² s ^-1	Cytoplasmic Diffusion	[42, 45]
	D _m	0.1 μ m ² s ^-1	Membrane Diffusion	[47]
PKC
	[P K C]	0.5 μ M	Concentration	[49]
	k b pkc	140 s ^-1	Binding	[23]
	k u pkc	1 s ^-1	Unbinding	[23]
	k _Ca	0.4 μ M	Michaelis-Menten Ca²⁺	[23]
	D _c	5 μ m ² s ^-1	Cytoplasmic Diffusion	[44]
	D _m	0.1 μ m ² s ^-1	Membrane Diffusion	[47]
Ca²⁺
	τ	8 s	Relaxation time	[23]
	[C a ²⁺]_o	0.1 μ M	Rest concentration	[48]